P29 - Neurociencia

EVs and their administration in a chronic stress model

Donsanti Catalina - Trumper Martín - Diaz Reyes Mercyleidi - Delgado Ocaña Susana - Banchio Claudia

Instituto de Biología Molecular y Celular, CONICET - UNR, Rosario, Santa Fe, Argentina
Contacto: donsanti@ibr-conicet.gov.ar

Neural stem cells (NSCs) possess the ability to self-renew and differentiate into the major neural lineages: neurons, astrocytes, and oligodendrocytes. This fate decision is finely regulated by both intrinsic signaling pathways and extrinsic cues from the surrounding microenvironment. In recent years, growing evidence has revealed that extracellular vesicles (EVs) mediate intercellular communication. We demonstrated that NSC-derived EVs increase NSCs differentiation towards the neuronal lineage. In addition, our results indicate that NSC-EVs support neuronal development even under oxidative stress and inflammation conditions, highlighting their potential in regenerative strategies when the cellular environment is compromised. In this work, we explore the underlying mechanisms of the above-mentioned effects. Through a series of biochemical and cell biology experiments, we demonstrated that NSC-EVs enhance NSC differentiation into neurons without affecting astroglial differentiation. This effect is accompanied by an increased cell viability and proliferation. These results reinforce the notion that EVs carry molecular signals that modulate NSCs behavior and promote neurogenesis. They likely act activating specific intracellular pathways that favor neuronal lineage commitment and survival, highlighting their potential as modulators of neural development and regeneration.

Palabras clave: Extracellular Vesicles, Neuronal Differentiation, Neural Steam Cells