P25 - Neurociencia

Exosomes as mediators of neurodegeneration in Parkinson’s disease: a proteomic analysis

Diaz Reyes M*, Robert MC*, Banchio C

Laboratorio de Biología Molecular y Celular de Lípidos, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR) Ocampo y Esmeralda, Predio CONICET and Departamento de Ciencias Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000, Rosario, Argentina. * Equal contribution
Contacto: robert@ibr-conicet.gov.ar

Exosome-based approaches represent an emerging area in Parkinson’s disease (PD) theranostics research. These small extracellular vesicles, involved in intercellular communication, reflect the molecular content of their cells of origin and can be isolated from accessible biological fluids such as blood, saliva, urine, and cerebrospinal fluid. Since PD diagnosis currently relies mainly on clinical evaluation of motor symptoms, it is often detected at advanced stages, when significant dopaminergic neuron loss has already occurred. The lack of objective biomarkers also complicates disease progression monitoring and the assessment of treatment efficacy. In this context, exosomes have emerged as a promising source of non-invasive biomarkers, potentially useful for early diagnosis and disease tracking. In this study, we used exosomes derived from cell lines overexpressing alpha-synuclein, a key protein in PD pathogenesis, to evaluate their effect on cell viability. We also performed a quantitative proteomic analysis using mass spectrometry to identify distinctive protein profiles in pathological exosomes compared to controls. These results could contribute to a better understanding of the role of exosomes in PD pathophysiology and highlight their potential as diagnostic, therapeutic, and monitoring tools.

Palabras clave: EXOSOMES, PARKINSON DISEASE, PROTEOMIC